Category: placenta

Polydeoxyribonucleotide (PDRN): a safe approach to induce therapeutic angiogenesis in peripheral artery occlusive disease and in diabetic foot ulcers


Department of Clinical and Experimental Medicine and Pharmacology, Section of Pharmacology, University of Messina, 98125, Messina, Italy.


Peripheral arterial occlusive disease (PAOD) of lower extremities is becoming more prevalent worldwide. The general prognosis is particularly negative with a high prevalence of coronary heart disease and cerebrovascular disease. Diabetic foot ulcers occur in 15% of all the patients with diabetes and proceed to lower-leg amputations. In diabetic ulcers, wound healing is impaired because of delayed angiogenesis. In both pathological conditions, therapeutic angiogenesis using angiogenic growth factors, particularly Vascular Endothelial Growth Factor VEGF, is expected to be a valuable treatment. The most used approaches are based on VEGF local delivery or gene therapy, but they failed to meet the expected primary goals of therapy. Adenosine receptor stimulation can induce VEGF expression in many types of cells and this may be achieved by stimulating the A(2A) or A(2B) receptor or both, following the signalling pathways activated by hypoxia. Polideoxyribonucleotide (PDRN) is obtained from sperm trout by an extraction process. The compounds hold a mixture of deoxyribonucleotides polymers with chain lengths ranging between 50 and 2000 bp. PDRN is able to stimulate VEGF production during pathological conditions of low tissue perfusion. It likely acts through the stimulation of A(2A) receptors. Furthermore, acute and chronic toxicity studies showed a good safety profile. PDRN has been shown to be effective in an experimental model of PAOD, hind limb ischemia, impaired wound healing and burn injury. Preliminary studies and ongoing clinical trials predict a significant therapeutic efficacy in patients. These data lead to hypothesize a role for PDRN in therapeutic angiogenesis.

[PubMed – indexed for MEDLINE]


The effect of human placenta extract in a wound healing model.


Yonsei University, Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Seoul, Korea.


Human placenta had been used on wound healing such as burns, chronic ulcers, and skin defects. Recently, human placenta has been widely used in the form of human placental extracts (HPE) by clinical field. However, it is unclear what the effect of HPE is on wound healing. We studied the effect and mechanism of HPE on wound healing.In this study, 10 mice (imprinting control region mice, 5 week old males, 30 g) were divided into an experimental group and a control group. An 8-mm diameter single full-thickness skin defect was made on the back by skin punch biopsy. At least 2.0 x 10 mL/30 g HPE was injected into the boundaries of the wound. Wound size measurements were taken by digital image every 3 days over 2 weeks. Hematoxylin and eosin (H and E), transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF), and CD31+ immunohistochemical stains were performed on the 6th and 14th day.The experimental group showed acceleration in the decrease of wound size compared with the control group from the third day to the ninth day. TGF-beta on the 6th day showed a statistically significant increase in the experimental group. VEGF on the 14th day showed a statistically significant increase in the experimental group. CD31+ was increased in the experimental group as wound healing progressed, but this increase was not statistically significant. The total number of vessels increased in the experimental group, but this was not statistically significant.We conclude that administering HPE directly to a wound margin promoted wound healing. This mechanism appears to be related to an increase in TGF-beta in the early phase of wound healing and VEGF in the late phase.

[PubMed – indexed for MEDLINE]

A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities.


Cellular Biochemistry Laboratory, Indian Institute of Chemical Biology, Dermatology Section, Institute of Child Health, Calcutta, India.



The authenticity of various prototype human placental extracts with biological activity, such as that inducing vitiligo repigmentation, is under serious criticism, mainly due to a lack of demonstration at the cellular level. Considering the present worldwide scenario with regard to the occurrence and treatment of vitiligo, a thorough scientific exploration of such extracts should be undertaken.


One such prototype placental preparation was prepared, and was evaluated with regard to its melanogenic action in C57BL/6J mice in vivo and its mitogenic and melanogenic activity on B16F10 mouse melanoma cells and normal human melanocytes in vitro. The extract was applied topically to mice with age-induced prolonged telogenic phase of hair growth (grey body coat hair). Standard 3H-thymidine incorporation and spectrophotometric methods were followed to illustrate mitogenic and melanogenic effects at the cellular level.


The resurgence of blue skin, followed by shiny black hair, at the regions of application of the extract demonstrated the reversal of the age-induced prolonged telogenic phase of hair growth to the anagenic phase after topical application of the extract on C57BL/6J mice. Further support was obtained from histology where, at the extract-treated sites, the development of new melanogenic centers and hair follicles was observed. During in vitro studies, the vehicle-free extract constituents stimulated both mitogenesis and melanogenesis of B16F10 mouse melanoma cells in a concentration-dependent manner. The cell morphology and extent of melanogenesis also showed significant changes. In addition, two known melanocyte activity-modulating peptides, endothelin-1 (ET-1) and adrenocorticotropic hormone (ACTH), were determined in the extract, chiefly in the total lipid fraction, indicating their effective cutaneous permeation.


The extract was found to be a potent mitogen in the in vitro condition and a potent melanogen in both the in vitro and in vivo situations. This strongly suggests its therapeutic potential for the repigmentation of vitiligo patches.


What are growth factors?

A growth factor is a naturally occurring substance capable of stimulating cellular growth, proliferation and cellular differentiation.  Usually it is a protein or a steroid hormone. Growth factors are important for regulating a variety of cellular processes.

Growth factors typically act as signaling molecules between cells. Examples are cytokines and hormones that bind to specific receptors on the surface of their target cells.  (

What does that mean?

Growth Factors are responsible for signaling our cells to make changes in our body, usually when repairing damaged cells or replacing dead cells.

How many growth factors are in the human placenta?

The human placenta contains potent amounts, over 128, rich growth factors.  Recent scientific research has shown that placenta is a rich healing and development agent for the body.  Inside the womb these growth factors are responsible for triggering cell mitosis (cell division) or the making of new cells.  Because the faetus grows at such a rapid rate many growth factors are needed to facilitate the growth process of the unborn baby’s organs, tissue, nerves, bones and the brain in such a short period of time.

Are growth factors necessary to heal after birth?

Yes, growth factors will play a large role in the bodies healing process after birth, however as we age our bodies produce less and less of these growth factors slowing our bodies natural regeneration processes.  As well, new mothers will be passing most of their body’s important nutrients to their new baby through the breast milk, leaving little nutrients for themselves. When the placenta is consumed after birth the rich growth factors give direct attention to damaged or developing tissue in the body, most importantly uterine, vaginal and breast tissue.  We believe these growth factors have a direct connection with the almost immediate slowing of blood loss after consumption of the raw placenta smoothie.  Mammals who consume the placenta never bleed after birth because they consume their entire placenta raw giving the body a large boost of the growth factors needed to heal the body completely and immediately.

What research has been done to prove these effects?

Mitogenic action of cytokines from placenta are shown to have physiological affects on the body including anti-inflammatory properties, regulation of the autonomous system, improvement of blood circulation, wound tissue healing, inhibition of protease, enhancement of nerve generation, balancing multiple hormone levels, immune boosting, analgesic effect and improvement of intestinal environment. (MFIII Human Placenta Injection, 2009)

Around the world many pharmaceutical companies are researching the benefits of using growth factors from human or animal placenta to aid in tissue regeneration.  MFIII Human Placenta Injection is sold on the Internet for a heavy price giving the public a chance to benefit from placenta extract for a wide range of health problems.  Famous footballers from UK premiership teams are being flown to countries like Switzerland and Serbia to have placenta injections to aid in the recovery process after serious injuries.  Placenta extract is also used in high end face creams and anti-aging balms and serums.

Research into placenta extract and the benefits of using growth factors for medicinal purposes is still ongoing however the benefits of using the placenta outside of the womb are now clear and only proves more why new mothers should consider keeping their rich organ for themselves.  After all, do we really know what the hospitals do with unwanted placentas???


5×2 ml – $50 – BUY FROM US

PLACENTREX by Albert David Pharmaceuticals


Anti-bacterial, Immunotropic / Wound Healer, Tissue / Vascular regenerator, Anti-inflammatory, Skin whitening, Rejuvenating, Tissue regenerating, Skin smoothing, Increased oxygen uptake and endurance, stamina

1-2 amps weekly recommended as an anti-age therapy

Each ml. of Placentrex is derived from 0.1 gm. of fresh term, sterilized, infection-free human placenta. Total Nitrogen content not more than 0.08% Preservative Benzyl Alcohol I P 1.5% w/v.
DNA, RNA, Nucleotides, Amino Acids, Peptides in natural form.

Placenta has been accepted as an immunologically privileged organ (graft rejection rare in pregnancy). Moreover, Polydeoxy Ribonucleotide (PDRN) present in Placental extract possesses anti-inflammatory effect. PDRN also helps release of growth factor from Platelets thus inviting Fibrinogenesis and Collagenesis.

It also promotes neovascularisation and epithelialisation and thus exerts excellent wound healing properties.

PDRN also possesses an immunomodulatory action.


Anti-inflammatory: Pelvic Inflammatory Diseases and Ectopic pregnancy


Wound healing: Bedsores, Diabetic Ulcer/Non-Healing ulcer/Varicose Ulcer, Post surgical wound repair in Caesarian sections,Hysterectomy, Oophorectomy.


Placentrex provides stimulating effect on cell renewal of the epidermis on the entire body, by promoting the formation of new blood vessels, and nerves, thus leading to healthy looking skin. Skin will appear young and vibrant.

1. Physical and physic fatigue. Placentrex helps to improve a general lack of vitality, concentration, eyesight and mental weakness.

2. Placentrex helps to prevent and regulate problems associated with aging such as high blood pressure, diabetes, hypertension, cholesterol, gastric ulcers, migraine, blood circulation and arthritis.

3. Premature aging and wear of different organs brain, heart, lungs, lver, kidneys and digestive system. Placentrex promotes tissue respiration.

4. Placentrex stimulates normal growth, regenerates and accelerates repair, of aged or injured muscle, skin, collagen, bone cartilage and nerve tissue.

5. Epidermal problems, such as wrinkles, decolorization, pigmentation, eye-bags and dull skin tone.

6. Anemia. Placentrex helps normalize blood characteristics.

7. After surgery, Placentrex promotes wound healing and reduces recovery time.

8.After effects of illness, Placentrex helps replenish nutrients required by your body.

9. Menopausal syndrome, Placentrex is proven to be highly effective for women in their mid 40’s, approaching menopause. Placentrex reduces the pain and changes associated menopause.

10. Patients undergoing chemotherapy for cancer treatment. Placentrex helps to improve physical fatigue, loss of appetite and immune system.